pmxs oct3 Search Results


pmxs oct3 4  (Addgene inc)
93
Addgene inc pmxs oct3 4
Pmxs Oct3 4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmxs oct3 4/product/Addgene inc
Average 93 stars, based on 1 article reviews
pmxs oct3 4 - by Bioz Stars, 2026-02
93/100 stars
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addgene pmxs oct3  (Addgene inc)
90
Addgene inc addgene pmxs oct3
(A) Correlation between GFP+ colony number and percentage 12 days post transduction. Each dot represents a single well of the reprogramming experiments. (B) Representative FACS plot for <t>SSEA1/Oct4-GFP</t> at 12 days post-viral transduction. (C, D) Relative reprogramming efficiencies are shown, with the fold changes indicated. (C) Fully reprogrammed efficiency, assessed by the percentage of SSEA1+Oct4-GFP+ cells; (D) Intermediately reprogrammed efficiency, assessed by percentage of SSEA1+Oct4-GFP- cells; Student's t-test (two-tailed) is used for statistics. Error bars, standard error. n = experiments with independent MEFs. Ctrl, wild type or heterozygous littermate controls; TKO, triple knockout of piwi.
Addgene Pmxs Oct3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/addgene pmxs oct3/product/Addgene inc
Average 90 stars, based on 1 article reviews
addgene pmxs oct3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


(A) Correlation between GFP+ colony number and percentage 12 days post transduction. Each dot represents a single well of the reprogramming experiments. (B) Representative FACS plot for SSEA1/Oct4-GFP at 12 days post-viral transduction. (C, D) Relative reprogramming efficiencies are shown, with the fold changes indicated. (C) Fully reprogrammed efficiency, assessed by the percentage of SSEA1+Oct4-GFP+ cells; (D) Intermediately reprogrammed efficiency, assessed by percentage of SSEA1+Oct4-GFP- cells; Student's t-test (two-tailed) is used for statistics. Error bars, standard error. n = experiments with independent MEFs. Ctrl, wild type or heterozygous littermate controls; TKO, triple knockout of piwi.

Journal: PLoS ONE

Article Title: PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells

doi: 10.1371/journal.pone.0097821

Figure Lengend Snippet: (A) Correlation between GFP+ colony number and percentage 12 days post transduction. Each dot represents a single well of the reprogramming experiments. (B) Representative FACS plot for SSEA1/Oct4-GFP at 12 days post-viral transduction. (C, D) Relative reprogramming efficiencies are shown, with the fold changes indicated. (C) Fully reprogrammed efficiency, assessed by the percentage of SSEA1+Oct4-GFP+ cells; (D) Intermediately reprogrammed efficiency, assessed by percentage of SSEA1+Oct4-GFP- cells; Student's t-test (two-tailed) is used for statistics. Error bars, standard error. n = experiments with independent MEFs. Ctrl, wild type or heterozygous littermate controls; TKO, triple knockout of piwi.

Article Snippet: The following plasmids were obtained from Addgene: pMXs-Oct3/4-IP (15918), pMXs-Sox2-IP (15919), pMXs-Klf4-IP (15920) and pMXs-c-Myc-IP (16921). (Addgene).

Techniques: Transduction, Two Tailed Test, Triple Knockout

(A) iPS colonies exhibited typical ES cell morphology and expressed Oct4-GFP homogeneously. (B) Representative figure showing TKO iPS cells expressed comparable level of Oct4-GFP as Ctrl cells. ( n = 20). Relative expression levels of (C) Oct4-GFP and (D) SOX2 proteins, as denoted by quantitative mean fluorescence intensity (MFI) shown by FACS analysis ( n = 3 and 8, respectively). (E) Both TKO and Ctrl cells remained pluripotent and express Oct4-GFP + SSEA1 + (>99%) over 30 passages. (F) A competition strategy was designed to determine if Piwi depletion compromises iPSC self-renewal. GFP+ cells (marked the iPSCs) were mixed in a 1-to-1 ratio with normal mouse ESCs (GFP-) cells, and cultured in the presence of LIF. With each passage the ratio of GFP/total cells was measured by FACS. The proportion of GFP+ cells with TKO were indiscernible from Ctrl cells over five passages (n = 3). Ctrl, wildtype or heterozygous littermate controls; TKO, triple knockout of piwi.

Journal: PLoS ONE

Article Title: PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells

doi: 10.1371/journal.pone.0097821

Figure Lengend Snippet: (A) iPS colonies exhibited typical ES cell morphology and expressed Oct4-GFP homogeneously. (B) Representative figure showing TKO iPS cells expressed comparable level of Oct4-GFP as Ctrl cells. ( n = 20). Relative expression levels of (C) Oct4-GFP and (D) SOX2 proteins, as denoted by quantitative mean fluorescence intensity (MFI) shown by FACS analysis ( n = 3 and 8, respectively). (E) Both TKO and Ctrl cells remained pluripotent and express Oct4-GFP + SSEA1 + (>99%) over 30 passages. (F) A competition strategy was designed to determine if Piwi depletion compromises iPSC self-renewal. GFP+ cells (marked the iPSCs) were mixed in a 1-to-1 ratio with normal mouse ESCs (GFP-) cells, and cultured in the presence of LIF. With each passage the ratio of GFP/total cells was measured by FACS. The proportion of GFP+ cells with TKO were indiscernible from Ctrl cells over five passages (n = 3). Ctrl, wildtype or heterozygous littermate controls; TKO, triple knockout of piwi.

Article Snippet: The following plasmids were obtained from Addgene: pMXs-Oct3/4-IP (15918), pMXs-Sox2-IP (15919), pMXs-Klf4-IP (15920) and pMXs-c-Myc-IP (16921). (Addgene).

Techniques: Expressing, Fluorescence, Cell Culture, Triple Knockout